DD-001

DD-001 is a drug delivery method employing biodegradable nanoparticles.  This formulation chemistry can be used to package nucleic acids, peptides, and small molecules for sustained release when applied in-vivo. 

 

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Transmission electron micrscopy (TEM) of nanoparticle formulation. A) TEM micrograph (Magnification 7000x) of PLGA nanoparticles.  The particles had an average size of 99 ± 44 nm. B) (Magnification 7000x) shows a TEM micrograph of oleic acid-coated SPIONs incorporated into the PLGA microparticles.  These composite particles had an average size of 85 ± 32 nm.  The oleic acid-coated SPIONs appeared to range in size from 5 to 15 nm yet incorporation of these SPIONs did not influence the size of the PLGA particles.  C) (Magnification 70,000x) PLGA/SPION nanoparticle.  The SPIONs have slightly aggregated inside the polymer, although this is difficult to determine since particles separated in the z-direction will appear aggregated in this 2-D projection.  When a lower concentration of magnetite was used (D) (1 mg / ml vs. 5 mg / ml in C) in the formation of the PLGA microparticles, fewer magnetite particles were incorporated into the polymer and their appeared to be no aggregation (Magnification 40,000x).

 

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Scanning electron microscopy (SEM) of nanoparticle formulation. SEM (Magnification 7,000x) of PLGA nanoparticles zeta potential = -15.62 mV

 

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Rapid and efficient transfect of neuronal cells with DD-001 nanoparticles. Confocal Images (63x) of mouse neuronal cells incubated for 1 hour with PLGA nanoparticles loaded with Coumarin 6.  Each image represents a 1.5 um section through the cell