CLT-008

CLT-008 is a naturally occuring peptide with potent anti-angiogenic activity. 

 

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CLT-008 Reduces Retinal Vascular Permeability in a rat model of Oxygen-induced Retinopathy

CLT-008 dose-dependent reduction of vascular permeability in OIR rat retina.  Rats received an intravitreal injection of CLT-008 in the right eye and PBS in the left eye at age P14.  Retinal vascular permeability was measured at P16 using the Evans blue method and normalized by total protein concentrations in the retina.  Permeability was expressed as percentage of the contralateral control (mean±S.D., n=4). 1, age-matched normal rats with a PBS injection; 2, OIR rats with a PBS injection; 3, 4 and 5, OIR rats with an injection of 1.875, 3.75 and 7.5 μg/eye of LT-008, respectively.

 

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CLT-008 downregulates VEGF following intravitreal administration

CLT-008 mediated downregulation of VEGF expression in the retinas.  OIR rats (P14), STZ-diabetic rats (2 weeks of diabetes) and normal adult rats received an intravitreal injection of angiostatin (7.5 μg/eye) in the right eye and PBS in the left eye.  Retinal VEGF levels were determined by Western blot analysis using an anti-VEGF antibody 1 day after the injection.  The same membranes were striped and reblotted with the anti-β-actin antibody (upper panels).  Each blot is a representative of results from three rats in each group. VEGF levels were semiquantified by densitometry, normalized by β-actin levels and expressed as percentage of control (lower panels). PBS, PBS-injected eye; Ang, angiostatin-injected eye; ns., not statistically significant.